Abstract:Objective：To investigate the effect of expression of pituitary tumor transforming gene(PTTG) on the expression of inhibitory of vascular endothelial growth factor(VEGF). Methods：The constructed recomibinant vector, pcDNA3.1-PTTGas that contained full-length antisense PTTG,was transfected into the cholangiocarcinoma cell line QBC939 in different quantity. After 48h incubation, the variation of expressions of PTTG and VEGF mRNAs and proteins were observed by RT-PCP and Western-blot method. Results：After successful transfecting the recomibinant vector into QBC939, the mRNAs and proteins of PTTG and VEGF were all inhibited. Linear correlation analysis showed positive correlation between descendent extents of PTTG and VEGF（P <0.05）. Conclusions：Antisense blocking PTTG gene can inhibit the expression of VEGF in both mRNA and protein levels, and a positive correlation between descendent extents of PTTG and VEGF was observed.