Abstract:Objective:To explore the effects of selenium enriched Spirulina platensis (Se-SP) on antioxidation and regeneration of rat hepatocytes after partial hepatectomy. Methods:Sixty-seven percent hepatectomy was performed in rats after high dose(H) and low dose(L) Se-SP was given via gastric tube for 7 days. Then selenium (Se), glutathione peroxidase (GPx), superoxide dismutase (SOD) and malondiaoldehyde(MDA) in hepatocytes were determined. The results were compared with normal saline giving via gastric tube before hepatectomy(C) and sham operation(S) groups.Hepatocytes were obtained before operation and 24h after operation,and the expression index of proliferating cell nuclear antigen (PCNA) in hepatocytes was detected by immunohistochemistry, and the DNA damage of hepatocytes induced by peroxide was analyzed by comet assay. Results:After partial hepatectomy, Se content, GPx and SOD activity, and PCNA expression index were obviously higher (P<0.05), whereas content of MDA was significantly lower (P<0.05) in hepatocytes of rats with high dose and low dose Se-SP(150mg/100/d) than those in control. The DNA oxidation damage in hepatocytes induced by peroxide (100 nM for 30 min) was decreased also in rats with high dose and low dose of Se-SP treatment. Conclusions:Se-SP has definite antioxidation and regeneration enhancing effects on rat hepatocytes after hepatectomy.