Abstract:Objective：To study the effect of antisense RNA retroviral vector of mTOR on the proliferation of vascular smooth muscle cells (VSMC). Methods：The conservative region of mTOR gene was inserted into pLXIN reversedly, then the vector was packaged in PT67 cells by transfection with lipofectamine and transfected to VSMC. The efficiency of antisense inhibition was verified，and the changes of p70S6k and 4E-BP1 were also determined at the same time. The proliferation activity was determined by flowcytometry and MTT. Results：After the vector was successfully transfected to the cells. pLXIN-A-mTOR vector could efficiently decrease mRNA and protein expression of mTOR and p70S6k of the cell, while the expression of 4E-BP1 increased significantly. The cell cycle and proliferation activity of VSMC was also stunned in phase G1. Conclusions：The mTOR antisense retroviral vector, pLXIN-A-mTOR, has been constructed successfully, which can significantly inhibit the proliferation of VSMC.