Abstract:Objective：To identify the anticoagulation effect of pcDNA3.1 transfected exogenous human throbomodulin molecule (hTM) on umbilical vein endothelial cells (HUVECs). Methods：The pcDNA3.1/hTM plasmid was transduced into HUVECs by cationic liposome. Expression of hTMmRNA and hTM protein was detected by RT-PCR and immunohistochemistry,respectively. Results：The expression of hTMmRNA in HUVECs transduced with pcDNA3.1/hTM was 1.7 times higher than cells without transfected PcDNA3.1/hTM. Immunogistochemical examination showed about 10% cell tranfected with PcDNA3.1/hTM and high-level hTM protein was detected on positive cells. Conclusions：The pcDNA3.1/hTM plasmid could be transfected into endothelial cells by cationic liposome and the exogenous hTM protein had complete anticoagulation biological activity.