Abstract:Objective：To explore the effect of HBV X gene in the expression of RhoC gene in HEPG2 cell line. Methods：The whole-length X gene was directly cloned to pcDNA3.1 vector.The recombinant vector(pcDNA3.1-X) was transfected into HEPG2 cells after selection with hygromycin,steady clones(HEPG2-X cells) were obtained.The expression of RhoC protein was analysed with immunohistochemical stain. Results：The recombinant plasmid could steadily express X protein efficiently, and the expression of RhoC protein in HEPG2-X cell was enhanced. Conclusions：HBV X gene can enhance the expression of RhoC protein in vitro. It may be related to invasiveness and metastasis of hepatocellular carcinoma.