Abstract:Abstract:Objective
To study the expression of BRMS1mRNA in human breast cancer tissues and their significance.
Methods The expression of BRMS1mRNA was detected by reverse transcriptionpolymerase chain reaction(RTPCR) in 71 breast cancer tissues and adjacent breast tissues,12 patients with benign breast tumors and 12 patients with normal breast tissue,and semiquantitative analysis of band densities was also performed.
Results The expression of BRMS1mRNA in 71 patients with breast cancer and adjacent breast tissue was 0.378±0.046 and 0.918±0.044, respectively; the expression of BRMS1mRNA in 12 patients with benign breast tumors and 12 patients with normal breast tissue was 0.908±0.047 and 0.934±0.028 respectively. BRMS1mRNA expression was significantly lower in breast cancer tissue compared to adjacent breast tissue,benign breast tumors and normal breast tissue (P<0.01) .The expression of BRMS1mRNA in breast cancer was reduced and had no relation with patient age,tumor size,and ER,PR (P>0.05), but was related to axillary lymph node metastasis and clinical stage(P<0.05). Conclusions The expression of BRMS1mRNA in patients with breast cancer was correlated with axillary lymph node metastasis and clinical stage, and the reduction of BRMS1mRNA expression could be an index for metastasis and prognosis of patients with breast cancer.