Abstract:Objective:To compare the expression difference of heat shock protein 70 (HSP70) in blood of acute cholecystitis (AC) patients before and after laparoscopic cholecystectomy, and to explore the relationship between HSP70 and AC at molecular and proteinic level.
Methods :Subjects in the study included 85 in-hospital AC patients who received laparoscopic cholectectomy. Blood before and after laproscopic operation were obtained. Ezyme linked immunosorbent assay (ELISA) was used to determine the quantity of HSP70. Four subjects were randomly selected and the total RNA and protein in blood were extracted. RT-PCR and Western-blotting were applied to determine the expression of HSP70. The data on the figure was analysed with the software Quantity one of Bio-rad Company.
Results:The expression level of HSP70 of acute cholecystitis patients before laparoscopic cholecystectomy (181.42±13.58) was significantly higher than that of those patients after laparoscopic cholecystectomy (63.74±9.03) (P<0.05). The results of RT-PCR and Western-blotting showed that the HSP70 expression level of acute cholecystitis patients decreasd notably after laparoscopic operation.
Conclusions:HSP70 is related closely with acute cholecystitis and it′s expression level increases when stimulated by acute inflammation. The expression level of HSP70 rapidly declines when the inflammatory focus is eliminated by laparoscopic operation.