五羟黄酮调节肝癌HepG2细胞bax基因表达诱导细胞凋亡的实验研究
作者:
通讯作者:
作者单位:

作者简介:

张继红

基金项目:


Quercetin regulates bax gene expression and induces apoptosis in hepatocellular carcinoma HepG2 cells
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 音频文件
  • |
  • 视频文件
    摘要:

    目的:探讨三磷酸肌醇( IP3) 和bax基因表达变化在五羟黄酮(Quercetin)诱导肝癌细胞凋亡中的作用。
    方法:以肝癌HepG2 细胞培养72 h为对照,以20,40,60,80μmol/L Quercetin作用于 HepG2 细胞72 h 时和60μmol/L Quercetin 作用于 HepG2 细胞 6,12,24,48,72 h,应用同位素试剂盒检测细胞IP3含量,RT-PCR分析bax mRNA表达,Western blotting 分析细胞bax蛋白表达,流式细胞仪检测细胞凋亡率。
    结果:各浓度的Quercetin作用于肝癌HepG2 细胞72 h,IP3含量显著低于对照组(P<0.01),bax mRNA和bax蛋白表达显著高于对照组,细胞凋亡率显著高于对照组(P<0.01); 60 μmol/L Quercetin 作用于肝癌HepG2 细胞6,12,24,48,72 h,各时相IP3含量显著低于对照组(P<0.01);12 h后bax mRNA和bax蛋白表达显著高于对照组,24 h后各时相细胞凋亡率为显著高于对照组(P<0.01)。
    结论:Quercetin能减少IP3生成,上调bax基因表达,诱导肝癌细胞凋亡。

    Abstract:

    Abstract:Objective:To explore the role of 1, 4, 5-trisphosphate inositol ( IP3) and bax gene expression in apoptosis of HepG2 cells induced by Quercetin.
    Methods :HepG2 cells were cultured for 72h as control. HepG2 cells were treated with different concentrations including 20,40.60,80μmol/L Quercetin for 72h, and treated with 60μmol/ L Quercetin for 12h, 24h, 48h and 72h. IP3, bax mRNA, bax protein and apoptosis rate were assayed by IP3-[3H] Birtrak assay,RT-PCR, Western blotting and flow cytometry, respectively.
    Results:In HepG2 cells incubated with each of the concentrations of Quercetin for 72 h,IP3continent was lower than that of control(P<0.01),bax mRNA expression and bax protein expression was higher than that of control,and the apoptosis rate was higher than that of control; in HepG2 cells incubated with Quercetin for 6 h, 12 h, 24 h, 48 h, 72 h, IP3content was lower than that of control(P<0.01);bax mRNA and bax protein expression in groups incubated with 60 μmol/L Quercetin for 12h was higher than that of control, and the apoptosis rate in groups incubated with 60μmol/ L Quercetin for 24h, 48h and 72h was significantly higher than that in control (P<0.01).
    Conclusions:Quercetin induces apoptosis of HepG2 cells by reducing IP3production and up-regulating bax gene expression.

    参考文献
    相似文献
    引证文献
引用本文

马兴标, 张继红, 梁力建, 黄洁夫.五羟黄酮调节肝癌HepG2细胞bax基因表达诱导细胞凋亡的实验研究[J].中国普通外科杂志,2007,16(12):10-117.
DOI:10.7659/j. issn.1005-6947.2007.12.010

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2007-12-25