Abstract:Objective:To explore the relationship between survivin and chemoresistance of gallbladder cancer cell line (GBC-SD) cells after treatment with various concentrations of cisplatin. Methods:RT-PCR and Western blot were used to analyze the changes in expression levels of survivin mRNA and protein. GBC-SD cells were examined with MTT assay to determine drug sensitivity to 4 routine chemotherapeutic drugs. Results:In GBC-SD cells, the sensitivity sequence of those 4 drugs from high to low was DDP>ADM>5-FU>MMC. The level of survivin mRNA decreased in all the GBC-SD cells treated with three concentrations of cisplatin for one day. The survivin mRNA treated with 0.5μg/mL DDP decreased 10%, decreased 36% for 3μg/mL DDP，and decreased 28% for 6μg/mL DDP. In the third day, the survivin level treated with 0.5μg/mL DDP cells increased 22%, with 3μg/mL DDP increased 64%, but with 6μg/mL DDP decreased 34% compared with the first day. Similarly, survivin protein level increased 15% for those cells treated with 0.5μg/mL DDP after three days, increased 12% for 3μg/mL DDP, but decreased 80% for 6μg/mL DDP. Conclusions:A high expression of survivin was induced in GBC-SD by low concentration of cisplatin. Survivin may correlate with the chemoresistence of GBC-SD cells.