Abstract:Objective： To investigate immunoliposomal docetaxel in combination with radiation on the expression of apoptosis-related proteins in LoVo cell line.
Methods ：Immunoliposomal docetaxel was prepared by coupled CEA antibody. LoVo cells were treated with immunoliposomal docetaxel and irradiation (group A), or with liposomal docetaxel and irradiation (group B), or irradiation alone (group C). The apoptosis index (AI) was evaluated by TUNEL method. The expressions of proteins p53, Bcl-2 and Bax were detected by flow cytometry. Fas、FasL and survivin proteins were detected by immunohistochemical staining and were quantified using semi-quantitative analysis by image analysis system.
Results：AI in group A (43.6%) was Significantly higher than that in group B (17.7 %) and group C (16.8%)(P<0.01).but no significant difference between group B and group C (P>0.05). Bax, Fas and FasL expression in group A were significantly higher than those in group B, but survivin expression in group A was lower than thatin group B (P<0.01).
Conclusions：Different pathways are involved in promotion of apoptosis in cancer cells that undergo docetaxel treatment, and decetaxel can enhance LoVo cells radiosensitization.