Abstract:Objective：To investigate the effect of HSP 70 in the proliferation and apoptosis of esophageal cancer cell line Eca-109 by inhibiting HSP 70 expression with HSP 70 antisense oligonucleotides.
Methods ：After Eca-109 cells were transfected with HSP 70 antisense oligonucleotides, analysis of HSPEO mRNA and protein expression by RT-PCR and western blotting were made respectively, the proliferation, induction of apoptosis and cell cycle were detected.
Results：After transfection with HSP 70 antisense oligonucleotides, the expression of HSP 70 was partially blocked, the proliferation of Eca-109 cells was apparently inhibited，and its inhibitory rate was increased to 25.5 % at 48h and 35.4% at 72h respectively. Apoptosis rate induced by HSP 70 antisense oligonucleotides was obviously higher than that by HSP 70 sense oligonucleotides or untransfected group (P<0.05).
Conclusions：HSP 70 antisense oligonucleotides could not only inhibit the proliferation, but also induce the apoptosis of Eca-109 cells.