Abstract:Objective:To investigate the expression of Pin1 and cyclin D1 in human pancreatic carcinoma and to discuss its role in oncogenesis of pancreatic cancer.
Methods :The mRNA expression of Pin1 and cyclinD1 in pancreatic tumor tissues and corresponding adjacent nontumor tissues 27 patients was detected by the real-time quantitative reverse transcription-polymerase chain reaction (RQ-RT-PCR). The results of RQ-RT-PCR were analyzed by one- way ANOVA and Fisher′s exact probabilities test.
Results:Cyclin D1 and Pin1 were overexpressed at mRNA level in pancreatic carcinoma tissues compared with their adjacent nontumor tissues. Cyclin D1 overexpression were found in 14 of 20 pancreatic carcinoma tissue specimens and Pin1 overexpression in 13 of 20 carcinoma tissue specimens. The expression of cyclin D1 and Pin1 in pancreatic cystadenoma tissues was not different than that of corresponding adjacent nontumor pancreatic tissue. Pin1 overexpression positively correlated with an increase in cyclin D1 levels as shown by Fisher′s exact probabilities test. However, Pin1 and cyclin D1 expression was not correlated with clinical stage and pathological parameters.
Conclusions:The overexpression of Pin1 in pancreatic carcinoma tissues could promote cyclin D1 expression, which might be a critical event in oncogenesis of pancreatic carcinoma. Pin1 may play a key role in pancreatic carcinoma.