CD1D基因与B7-1基因共转染胰腺癌细胞及其抗癌作用的实验研究
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    王昆华


    Study of cotransfection of B7-1 gene and CD1D gene in pancreatic carcinoma cell and its anti-tumor responses in mice
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      目的:观察共转染小鼠B7-1和CD1D 基因对小鼠胰腺癌细胞的免疫应答。
      方法:将表达B7-1和CD1D 基因的逆转录病毒载体导入包装细胞系,然后转染入胰腺癌细胞。用PCR和Western方法鉴定细胞表达;用B7-1和CD1D 阳性的细胞在体内诱导抗肿瘤免疫反应。
      结果:B7-1和CD1D 阳性的细胞在同基因小鼠体内能诱导抗肿瘤免疫反应并抑制肿瘤生长。
      结论:B7-1基因和CD1D 基因共转染肿瘤细胞,能抑制肿瘤生长,可能为肿瘤的基因治疗开辟一条新途径。

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      Abstract:Objective:To study the cotransfection mB7-1 and mCD1D gene into pancreatic cancer cells of rats and to observe its anti-tmor responses.
      Methods :Recombinant retroviral vectors expressing mB7-1and mCD1D gene were packaged into GP2-293 cell lines and transfected. The expressions of mB7-1 and mCD1D were detected with PCR and Western blot. The positive cells of mB7-1 and mCD1D were used to induce the anti-tumor immunity in vitro.
      Results: Anti-tumor immunity was induced after B7-1 and CD1D positive cells were coinoculated in syngeneic mice. Furthermore, the growth of tumor was inhibited.
      Conclusions:Cotransfection of B7-1 and CD1D could induce anti-tumor effect. This study provide a foundation for the application of B7-1 and CD1D gene therapy in tumor.

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    王昆华, 龚昆梅, 张勇学, 钟鸣, 欧阳一鸣, 凌平,黄映光, 张剑, 朱宇, 刘为军, 赵喜荣. CD1D基因与B7-1基因共转染胰腺癌细胞及其抗癌作用的实验研究[J].中国普通外科杂志,2007,16(5):10-.
    DOI:10.7659/j. issn.1005-6947.2007.05.009

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    • 在线发布日期: 2007-05-25