Abstract:Objective:To investigate the inhibitory effect of prostaglandin E1 (PGE1) on production of collagen type Ⅰ and Ⅲ in liver of rabbit with schistosomia japonicum (SJ), and on expression of glucose regulatory protein/immunoglobulin heavy chain binding protein (Grp78/BiP).
Methods:Forteen rabbits were infected with cercaria of SJ by percutaneous patch. From 60th to 120th day after infection, 7 of them received PGE1 （2.5μg/kg.d-1）by intravenous injection. The mRNA expressions of collagen type Ⅰ and Ⅲ, as well as Grp78/BiP were detected by reverse transcription-polymerase chain reaction (RT-PCR).
Results:Up-regulated expressions of collagen Ⅰ,Ⅲ mRNA(14.81±3.57 and 12.9±3.25) were observed during the process of liver fibrosis in rabbit with schistosomiasis. Grp78/BiP mRNA increased (P<0.05). Expression of collagen Ⅰ,Ⅲ mRNA (compared with models, 5.79±1.26 and 5.34±1.08, P<0.01) had been attenuated markedly in the group treated with exogenous PGE1. Expression of Grp78/BiP was inhibited from 0.43±0.07 in models to 0.29±0.02 in treated group (P<0.05).
Conclusions:These data in experimental models strongly suggest that PGE1 can inhibite the maturation of collagen and thus, can effectually decrease hepatic collagen formation in down-regulated the expression of Grp78/BiP in rabbits with schistosomiasis.