Abstract:Objective:To establish a stable primary hepatocellular carcinoma(HCC) model of C57BL/6J mice, and establish a basis for indepth laboratory research in hepatocellular cancer.
Methods :Ninety C57BL/6J mice were induced to establish HCC models by combination of diethylnitrosamines (DEN), carbon tetrachloride (CCl4) and ethanol for twenty weeks, then the occurrence and development of HCC were observed, and other 10 C57BL/6J mice were used as normal control(no drug was given). RTPCR method was used to observe AFP mRNA expression in liver tissues and tumor tissues.
Results:seventyone of 90 mice developed HCC(78.9%), cirrhosis was observed in 11 of 90 mice(12.2%)， and 8 of them died of toxic hepatitis and acute hepatocellular necrosis. AFP mRNA was expressed only in liver cancer tissues. No obvious liver pathologic changes were observed in the control group. The histopathologic changes of HCC were high or middle differentiation.
Conclusions:An AFPsecreting C57BL/6J mouse liver cancer model was established by induction with combination of DEN, CC14 and ethanol in a relatively short time. The rate of cancerous change was high, and some of the hepatic changes included hepatitis and cirrhosis. This is a rather ideal animal model for the study of liver cancer.
匡志鹏, 谢裕安, 杨帆, 梁安民, 罗小玲, 吴继宁.12 C57BL/6J小鼠肝癌动物模型的建立[J].中国普通外科杂志,2007,16(7):12-.复制