Abstract:Objective:To establish a stable primary hepatocellular carcinoma(HCC) model of C57BL/6J mice, and establish a basis for indepth laboratory research in hepatocellular cancer.
Methods :Ninety C57BL/6J mice were induced to establish HCC models by combination of diethylnitrosamines (DEN), carbon tetrachloride (CCl4) and ethanol for twenty weeks, then the occurrence and development of HCC were observed, and other 10 C57BL/6J mice were used as normal control(no drug was given). RTPCR method was used to observe AFP mRNA expression in liver tissues and tumor tissues.
Results:seventyone of 90 mice developed HCC(78.9%), cirrhosis was observed in 11 of 90 mice(12.2%)， and 8 of them died of toxic hepatitis and acute hepatocellular necrosis. AFP mRNA was expressed only in liver cancer tissues. No obvious liver pathologic changes were observed in the control group. The histopathologic changes of HCC were high or middle differentiation.
Conclusions:An AFPsecreting C57BL/6J mouse liver cancer model was established by induction with combination of DEN, CC14 and ethanol in a relatively short time. The rate of cancerous change was high, and some of the hepatic changes included hepatitis and cirrhosis. This is a rather ideal animal model for the study of liver cancer.