Abstract:Objective To investigate the effect of phenylacetate (PA), a differentiation induce agent, on cell cycle and proliferation in colorectal carcinoma HCT-8 cell line.
Methods HCT-8 cells were grown in the presence of PA (1.0, 2.0, 3.0, 4.0, and 5.0mmol/L) for 24h, 48h and 72h, respectively. The cellular proliferation inhibitory ratio was evaluated by MTT assay. The different phases of the cell cycle were analyzed using flow cytometer.
Results HCT-8 cells were treated by 1.0-5.0 mmol/L PA for 24-72 h. With the increase of concentration of PA or the prolongation of the treatment time, the proliferation inhibitory ratio of the tumour cells increased notably by 5.1%-24.3% when treated for 24h, 16.7%-72.3% when treated for 48 h, and 30.2%-93.4% when treated for 72 h, respectively. When HCT8 cells were treated with 5.0 mmol/L PA for 72h, cell cycle analysis with flow cytometry showed that the percentage of HCT8 cells in G0/G₁ phase reduced significantly and S phase was relatively increased(P＜0.05).
Conclusions PA can arrest G₁ cell cycle and inhibit cell proliferation.