Abstract:Objective:To investigate the expression of stem cell factor Oct-4 in human cholangiocarcinoma QBC939 subclone cell lines with different metastatic capacities.
Methods :The screening and use of one subclone cell line with high metastasis capacity and one subclone cell line with low metastasis capacity was performed by the in vitro invasive assay. Then, the biologic characters of the two subclone cell lines were compared, analyzed and identified. The expression of the stem cell factor Oct-4 in the two selected subclone cell lines was determined by RT-PCR and Western-blot assays.
Results:Two subclones of QBC939 with different metastatic capacities, and named QBC-H and QBC-L were isolated，and their invasion indexes were 263±5.9 and 154±6.6, respectively (P<0.05）. The expression of mRNA and protein of Oct-4 was stronger in QBC-H than in QBC-L.
Conclusions:There is a close relationship between the expression strength of the stem cell factor Oct-4 and the metastatic capacity of Oct-4.The stem cell factor may play an important role in regulation of the metastatic capacity of cholangiocarcinoma.