Abstract:Objective:To study the effect of macrophage migration inhibitory factor(MIF) on cell proliferation and angiogenesis of human colon carcinoma.
Methods :Effect of different concentrations of Anti-human MIF antibody(Anti-MIF) on cell proliferative and expression of vascular endothelial growth factor(VEGF) of human colon carcinoma cell line HCT-116 cells was examined.
Results:After HCT-116 cells were exposed to Anti-MIF (100，200，400，and 800 μg/L)for 48 and 72 h, HCT-116 cells growth was strongly inhibited in a dose and time-dependent manner;After exposure of the cells to Anti-MIF (100，200，400，and800μg/L) for 72 h，FCM analysis showed the ratio of G0/G1 phase cells increased,and the ration of S and G2/M phase cells decreased. Comparison with the negative group, the expression of VEGF after the cells exposure to Anti-MIF for 72h were significantly decreased, and was in dose-dependent manner （P＜0.05）.
Conclusions:MIF participates can modulat the cell growth cycle and can affect tumor neovascularization via VEGF. MIF plays an important role in the pathogenesis of colon carcinoma.