Abstract:Objective:To investigate the effects of the ischemic postconditioning (IPO) on the ischemia reperfusion injury (IRI) in rat livers.
Methods :One hundred and tweenty Wistar rats were randomly divided into ischemia reperfusion group (IRI), ischemic postconditioning (IPO) group and sham operation (S) group. Liver tissues were harvested at 0, 0.5, 1, 2, 4, 8, 12 h and 24 h after reperfusion in both IPO and IRI groups. The mRNA expressions of the immediate early gene c-fos and c-jun were detected with RT-PCR methods.
Results:(1) The expression of c-fos and c-jun both maintained a high level from 0.5 h to 2 h after reperfusion in IRI group, and the peak time occurred at 1 h after reperfusion. After 4 hours of reperfusion, the c-fos expression began to decrease gradually while the c-jun expression still remained high.(2) At every time point, the c-fos expressions in IPO group were lower than IRI group (P>0.05).(3) Compared with IRI group, the c-jun expression at 0.5, 1 h and 2 h after reperfusion in IPO group was significantly lower (P<0.05).
Conclusions:IPO can impact the immediate early gene expression at the very early moment after reperfusion, which may be part of the mechanisms of IPO protection effect on ischemia reperfusion injury.