Abstract:Objective:To explore the selection of indicator to judge the severity of hepatic ischemic and reperfusion injury(IRI).
Methods :Local warm hepatic IRI model was established routinely in rat. The serum alanine aminotransferase(ALT) levels were determined and specimens were sent for pathologlical test at time of 0, 1, 2, 4, 6, 12, 24, 48 h of reperfusion, after ischemic time of 60 and 90 min respectively. The rats were re-grouped into 4 groups of different severity according to the grading systems for IRI used in our institute， and the relationship between ALT level and pathological changes was analyzed.
Results:After 60 min of ischemia time, there was mild IRI in 75%, intermediate IRI in 14.6% and severe IRI in 10.4% of rats, respectively. After 90 min of ischemia time, there was mild IRI in 41.7%, intermediate IRI in 25%,severe IRI in 16.7%, very severe IRI in 16.7% of rats respectively. The results showed that the more severe the degree of IRI, the higher rate of the cell death, and the higher of the ALT value.
Conclusions:The serum ALT level can directly reflect the severity of IRI. The more severe the degree of IRI, the higher was the ALT level, and the higher was the cell death rate.