Abstract:Objective:To explore the protective effect of portal vein administration of propofol on hepatic ischemia reperfusion injury (HIRI) and its mechanism.
Methods :Thirty-two male rabbits were randomly allocated into four groups:Group A (sham operation group), the abdomen was only opened and closed;group B,the hepatic inflow was occluded for 30 min,and reperfused for 60 min; group C,the same managment as group B + propofol injected through jugular vein;group D,the treatment same as group B + propofol injected through portal vein.Drug injection was completed 20 min before hepatic inflow occlusion. Serum ALT and AST, and endothelin-1 (ET-1) and nitric oxide(NO)in the hepatic tissue and blood, and the content of ATP in hepatic tissue were determined.
Results:The level of ET-1 in plasma and hepatic tissue was significantly increased in group B compared to group C and D (P＜0.01、P＜0.05). The level of NO in serum and hepatic tissue in group B was significantly lower than that in group C,D (P＜0.01). The content of ATP in hepatic tissue was significantly decreased in group B compared to group C and D (P＜0.01).Comparing the efficiency of administration routes, propofol delivered through portal vein have better protective effect on HIRI.
Conclusions:Propofol delivered through portal vein has significant protective effect on HIRI. Propofol may adjust disbalance between ET-1 and NO to improve microcirculation,and elevate the content of ATP in the hepatic tissue.All of these contribute to protective effect on HIRI.