Abstract:Objective:To study the effect and mechanism of adenovirus-mediated mda-7/IL-24 and/or adriamycin treatment of hepatoma in nude mice.
Methods :The recombinant adenovirus vector carrying melanoma differentiation-associated gene-7 (Ad-mda-7) and/or ADM were injected into the tumor-bearing mice. Their effects on the volume of the tumor and the survival rate of the mice were observed, and the apoptosis of cancer tissue was detected by TUNEL staining in each group.
Results:Ad-mda-7 was successfully constructed and expressed in vivo. Compared with the other three groups, the mice treated with Ad-mda-7 combined with adriamycin had longer average survival time (83.8±4.82) d (P<0.01); the average size of tumor in combined group was reduced significantly, the tumor inhibition rate was 79.78%, which was higher than the other groups (P<0.05). Enhanced induction of apoptosis by Ad-mda-7 combined with adriamycin was observed. The TUNEL-positive cells showed that apoptotic index in combination therapy group was 38.1%±4.2 %，which was higher than that of the other 3 groups (P<0.05).
Conclusions:Ad-mda-7 combined with adriamycin has stronger antitumor potency and has more synergistic effect on metastastic hepatoma mouse model than mda-7/IL-24 or adriamycin alone, and the mechanism is related to the promotion of apoptosis of tumor cells.